1btl

CRYSTAL STRUCTURE OF ESCHERICHIA COLI TEM1 BETA-LACTAMASE AT 1.8 ANGSTROMS RESOLUTION
(see also page TEM1-beta-Lactamase/beta-lactamase Inhibitor Protein (BLIP))



The enzyme TEM1 β-lactamase (EC 3.5.2.6; TEM1) and its protein inhibitor, β-lactamase inhibitor protein (BLIP) form a complex. Overlap of the residues which participate in TEM1-BLIP interactions between: 1) complex of mutated BLIP (F142A) and wildtype TEM1 (lime; 1s0w) ; 2) complex of the wildtype BLIP/wildtype TEM1 (magenta; 1jtg); 3) unbound wildtype BLIP (structure from Ref 2) and unbound wildtype TEM1 (1btl), these two structures colored orange ). T marks residues of TEM1 and B of BLIP. The distance between TEM Glu-104 and BLIP Lys-74 is marked for the wildtype (1jtg) and mutated (F142A, 1s0w) TEM1-BLIP complex structures.



The TEM1-BLIP complex where BLIP residues of the bound structure are colored blue and of the unbound are in cyan. TEM1 residues from the bound complex are in red and from the <font color='black'>unbound structure in yellow. <font color='blue'>BLIP and <font color='red'>TEM1 residues are labeled <font color='blue'>blue and <font color='red'>red. The <scene name='2b5r/Bound_unbound/4'>interface between BLIP and TEM1 was divided into six interface clusters (<scene name='2b5r/C1/2'>C1, <scene name='2b5r/C2/3'>C2 , <scene name='2b5r/C3/2'>C3 , <scene name='2b5r/C4/3'>C4 , <scene name='2b5r/C5/2'>C5 , <scene name='2b5r/C6/2'>C6 , 2b5r). Superpositions of these clusters from TEM1–BLIP (complex-1jtg), TEM1 (unbound-1btl) and BLIP (unbound, from Ref 2) structures are shown. <scene name='2b5r/C1mut/2'>Overlap of cluster C1 from TEM1-BLIP wt, mutant and unbound structures (<font color='red'>TEM1wt -<font color='blue'>BLIPwt complex, <font color='lime'>TEM1wt-BLIPD49A, <font color='black'>unbound TEM1 (yellow), and <font color='cyan'>unbound BLIP ). <font color='blue'>D49 in BLIP is located in the center of C1, surrounded by <font color='red'>4 TEM1 residues. The <font color='blue'>D49A mutation (i.e. removal of a side chain) does not cause structural change in the TEM1-BLIP complex.

About this Structure
1BTL is a 1 chain structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.